ACEDB documentation: CLASSIFICATION OF TWO-FACTOR DATA

# CLASSIFICATION OF TWO-FACTOR DATA

    CLASSIFICATION OF TWO-FACTOR DATA 	Jonathan Hodgkin 21/4/93
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       Phenotypes:  WT = wild type
                    nonWT = viable non-wild-type,
                    ALL = total viable progeny
                    X = x/x and xy/x+, Y = y/y and xy/+y
                    XY = xy/xy
                    D = d/+ and d/d, dx/+ etc
                    H = xy/x+

            Type            Phenotypes              Calculation

    A.  Recessive markers x and y, segregation from xy/++ hermaphrodites

        1.  Full            WT X Y XY           (2p-p^2)/2 = (X+Y)/(WT+X+Y+XY)

        2.  One_recombinant WT X                (2p-p^2)/3 = X/(WT+X)

        3.  Selected        X XY                (2p-p^2) = X/(X+XY)

        4.  One_all         X ALL               (2p-p^2)/4 = X/ALL

        5.  Recs_all        X Y ALL             (2p-p^2)/2 = (X+Y)/ALL

    A'   Recessive markers x and y, segregation from xy+/++z, where z is
            tightly linked and z/z is lethal or otherwise not scored.

        6.  One_let         X ALL               (2p-p^2)/2 = X/ALL

    B    Recessive markers, segregation from x+/+y hermaphrodites

        7.  Tested          H X                 2p/(1+p) = H/X

        8.  Selected_trans  X XY                p^2 = XY/(X+XY)

    C    Recessive markers, scored by backcross: xy/++ males crossed with
            xy/xy hermaphrodites.  Usually only males scored.

        9.  Backcross       WT X Y XY           p = (X+Y)/(WT+X+Y+XY)

       10.  Back_one        WT X                p = X/(WT+X)

    D    Recessive sex-linked markers, x+/+y hermaphrodites crossed with wild
            males, male progeny scored.

       11.  Sex_all         WT X Y XY           p = (WT+XY)/(WT+X+Y+XY)

       12.  Sex_one         WT X                p = WT/(WT+X)

    D'   Recessive sex-linked markers, xy/++ hermaphrodites crossed with wild
            males, male progeny scored.

       13.  Sex_cis         X ALL               p/2 = X/ALL

    E    One marker (d) is dominant or semidominant.  Segregation from x+/+d
            hermaphrodites.  For dom_semi, D = d/+, not d/d (as in sqt)

       14.  Dom_one         WT nonWT           (2p-p^2)/4 = WT/(WT+nonWT)

       15.  Dom_selected    WT X               (2p-p^2) = WT/(WT+X)

       16.  Dom_semi        XD ALL             (p-p^2)/2 = XD/ALL

    E'  One marker (d) is dominant, and either d or x is recessive lethal

       17.  Dom_let         WT ALL             (2p-p^2)/3 = WT/ALL

    F   Direct: single progeny picked and tested for genotype
            on both chromosomes

       18.  Direct          R = # recombinant chromosomes   p = R/T
                            T = # total chromosomes

    G   Complex:

       19.  Complex_mixed   X ALL             (2p-p^2)/5 = X/ALL

    H   Segregation from lethal heterozygote, x +/+ y, where y is lethal; WT
    	progeny picked, N = number of these failing to segregate x.

       20.  Lethal_tested	N WT		  (2p-p^2)/(2+p^2) = N/WT