TRNASCAN-SE(1)

NAME
tRNAscan-SE - detection of transfer RNA genes in genomic sequence

SYNOPSIS
tRNAscan-SE [-options] <FASTA file(s)>

DESCRIPTION
tRNAscan-SE 2.0.6 (May 2020) Copyright © 2019 Patricia Chan and Todd Lowe

    University of California Santa Cruz

Freely distributed under the GNU General Public License (GPLv3)

    Scan a sequence file for tRNAs

-- default: use Infernal & tRNA covariance models

    with eukaryotic sequences (use 'Search Mode Options' below to scan other types of sequences)

Search Mode Options:

-E  : search for eukaryotic tRNAs (default)

-B  : search for bacterial tRNAs

-A  : search for archaeal tRNAs

-M <model>
    : search for mitochondrial tRNAs options: mammal, vert

-O  : search for other organellar tRNAs

-G  : use general tRNA model (cytoslic tRNAs from all 3 domains included)

--mt <model>
    : use mito tRNA models for cytosolic/mito detemination (if not specified, only cytosolic isotype-specific model scan will be performed)

-I  : search using Infernal default use with -E, -B, -A, or -G; optional for -O

--max
    : maximum sensitivity mode - search using Infernal without hmm filter (very slow)

-L  : search using the legacy method (tRNAscan, EufindtRNA, and COVE) use with -E, -B, -A or -G

-C  --cove
    : search using COVE analysis only (legacy, extremely slow) default use with -O

-H  --breakdown
    : show breakdown of primary and secondary structure components to covariance model bit scores

-D  --nopseudo
    : disable pseudogene checking

Output options:

-o  --output <file>
    : save final results in <file>

-f  --struct <file>
    : save tRNA secondary structures to <file>

-s  --isospecific <file>
    : save results using isotype-specific models in <file>

-m  --stats <file>
    : save statistics summary for run in <file> (speed, # tRNAs found in each part of search, etc)

-b  --bed <file>
    : save results in BED file format of <file>

-a  --fasta <file>
    : save predicted tRNA sequences in FASTA file format of <file>

-l  --log <file>
    : save log of program progress in <file>

--detail
    : display prediction outputs in detailed view

--brief
    : brief output format (no column headers)

-? #
    : '#' in place of <file> chooses default name for output files

-p  --prefix <label>
    : use <label> prefix for all default output file names

-d  --progress
    : display program progress messages

-q  --quiet
    : quiet mode (credits & run option selections suppressed)

-y  --hitsrc
    : show origin of hits (Ts=tRNAscan 1.4, Eu=EufindtRNA, Bo=Both Ts and Eu, Inf=Infernal)

Specify Alternate Cutoffs / Data Files:

-X  --score <score>
    : set cutoff score (in bits) for reporting tRNAs (default=20)

-g  --gencode <file>
    : use alternate genetic codes specified in <file> for determining tRNA type

-z  --pad <number>
    : use <number> nucleotides padding when passing first-pass tRNA bounds predictions to CM analysis (default=8)

--len <length>
    : set max length of tRNA intron+variable region for legacy search mode (default=116bp)

Misc Options:

-h  --help
    : print this help message

-c  --conf <file>
    : tRNAscan-SE configuration file (default: tRNAscan-SE.conf)

-Q  --forceow
    : do not prompt user before overwriting pre-existing result files (for batch processing)

--match <EXPR>
    : search only sequences with names matching <EXPR> string (<EXPR> may contain * or ? wildcard chars)

--search <EXPR>
    : start search at sequence with name matching <EXPR> string and continue to end of input sequence file(s)

Special Advanced Options (for testing & special purposes)

-U  : search for tRNAs with alternate models defined in configuration file

-t  --tscan
    : search using tRNAscan only (defaults to strict params)

--tmode <mode>
    : explicitly set tRNAscan params, where <mode>=R or S (R=relaxed, S=strict tRNAscan v1.3 params)

-v  --verbose <file>
    : save verbose tRNAscan 1.3 output to <file>

--nomerge
    : Keep redundant tRNAscan 1.3 hits (don't filter out multiple predictions per tRNA identification)

-e  --eufind
    : search using Eukaryotic tRNA finder (EufindtRNA) only (defaults to Normal search parameters when run alone, or to Relaxed search params when run with Cove)

--emode <mode>
    : explicitly set EufindtRNA params, where <mode>=R, N, or S (relaxed, normal, or strict)

--iscore <score>
    : manually set "intermediate" cutoff score for EufindtRNA

-r  --fsres <file>
    : save first-pass scan results from EufindtRNA, tRNAscan, or Infernal hmm in <file> in tabular results format

--mid
    : fast scan mode - search using Infernal with mid-level strictness of hmm filter

-F  --falsepos <file>
    : save first-pass candidate tRNAs in <file> that were then found to be false positives by second-pass analysis

--missed <file>
    : save all seqs that do NOT have at least one tRNA prediction in them (aka "missed" seqs)

--thread <number>
    : number of threads used for running infernal (default is to use available threads)

AUTHOR
 This manpage was written by Nilesh Patra for the Debian distribution and
 can be used for any other usage of the program.