LOCUS       U00096                   840 bp    DNA     linear   BCT 11-MAR-2009
DEFINITION  Escherichia coli str. K-12 substr. MG1655, complete genome.
ACCESSION   U00096 REGION: 1..840
VERSION     U00096.2  GI:48994873
PROJECT     GenomeProject:225
DBLINK      Project:225
KEYWORDS    .
SOURCE      Escherichia coli str. K-12 substr. MG1655
  ORGANISM  Escherichia coli str. K-12 substr. MG1655
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (sites)
  AUTHORS   Kang,W.K., Icho,T., Isono,S., Kitakawa,M. and Isono,K.
  TITLE     Characterization of the gene rimK responsible for the addition of
            glutamic acid residues to the C-terminus of ribosomal protein S6 in
            Escherichia coli K12
  JOURNAL   Mol. Gen. Genet. 217 (2-3), 281-288 (1989)
   PUBMED   2570347
REFERENCE   2  (bases 1 to 840)
  AUTHORS   Blattner,F.R., Plunkett,G., Bloch,C.A., Perna,N.T., Burland,V.,
            Riley,M., Collado-Vides,J., Glasner,J.D., Rode,C.K., Mayhew,G.F.,
            Gregor,J., Davis,N.W., Kirkpatrick,H.A., Goeden,M.A., Rose,D.J.,
            Mau,B. and Shao,Y.
  TITLE     The complete genome sequence of Escherichia coli K-12
  JOURNAL   Science 277 (5331), 1453-1474 (1997)
   PUBMED   9278503
REFERENCE   3  (bases 1 to 840)
  AUTHORS   Riley,M., Abe,T., Arnaud,M.B., Berlyn,M.K., Blattner,F.R.,
            Chaudhuri,R.R., Glasner,J.D., Horiuchi,T., Keseler,I.M., Kosuge,T.,
            Mori,H., Perna,N.T., Plunkett,G. III, Rudd,K.E., Serres,M.H.,
            Thomas,G.H., Thomson,N.R., Wishart,D. and Wanner,B.L.
  TITLE     Escherichia coli K-12: a cooperatively developed annotation
            snapshot--2005
  JOURNAL   Nucleic Acids Res. 34 (1), 1-9 (2006)
   PUBMED   16397293
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 840)
  AUTHORS   Arnaud,M., Berlyn,M.K.B., Blattner,F.R., Galperin,M.Y.,
            Glasner,J.D., Horiuchi,T., Kosuge,T., Mori,H., Perna,N.T.,
            Plunkett,G. III, Riley,M., Rudd,K.E., Serres,M.H., Thomas,G.H. and
            Wanner,B.L.
  TITLE     Workshop on Annotation of Escherichia coli K-12
  JOURNAL   Unpublished
  REMARK    Woods Hole, Mass., on 14-18 November 2003 (sequence corrections)
REFERENCE   5  (bases 1 to 840)
  AUTHORS   Glasner,J.D., Perna,N.T., Plunkett,G. III, Anderson,B.D.,
            Bockhorst,J., Hu,J.C., Riley,M., Rudd,K.E. and Serres,M.H.
  TITLE     ASAP: Escherichia coli K-12 strain MG1655 version m56
  JOURNAL   Unpublished
  REMARK    ASAP download 10 June 2004 (annotation updates)
REFERENCE   6  (bases 1 to 840)
  AUTHORS   Hayashi,K., Morooka,N., Mori,H. and Horiuchi,T.
  TITLE     A more accurate sequence comparison between genomes of Escherichia
            coli K12 W3110 and MG1655 strains
  JOURNAL   Unpublished
  REMARK    GenBank accessions AG613214 to AG613378 (sequence corrections)
REFERENCE   7  (bases 1 to 840)
  AUTHORS   Perna,N.T.
  TITLE     Escherichia coli K-12 MG1655 yqiK-rfaE intergenic region, genomic
            sequence correction
  JOURNAL   Unpublished
  REMARK    GenBank accession AY605712 (sequence corrections)
REFERENCE   8  (bases 1 to 840)
  AUTHORS   Rudd,K.E.
  TITLE     A manual approach to accurate translation start site annotation: an
            E. coli K-12 case study
  JOURNAL   Unpublished
REFERENCE   9  (bases 1 to 840)
  AUTHORS   Blattner,F.R. and Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-1997) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
REFERENCE   10 (bases 1 to 840)
  AUTHORS   Blattner,F.R. and Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-1997) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
REFERENCE   11 (bases 1 to 840)
  AUTHORS   Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-1998) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
REFERENCE   12 (bases 1 to 840)
  AUTHORS   Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2004) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
  REMARK    Sequence update by submitter
REFERENCE   13 (bases 1 to 840)
  AUTHORS   Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2006) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
  REMARK    Protein updates by submitter
REFERENCE   14 (bases 1 to 840)
  AUTHORS   Rudd,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2007) Department of Biochemistry and Molecular
            Biology, University of Miami Miller School of Medicine, 118 Gautier
            Bldg., Miami, FL 33136, USA
  REMARK    Annotation update from ecogene.org as a multi-database
            collaboration
REFERENCE   15 (bases 1 to 840)
  AUTHORS   Rudd,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2007) Department of Biochemistry and Molecular
            Biology, University of Miami Miller School of Medicine, 118 Gautier
            Bldg., Miami, FL 33136, USA
  REMARK    Annotation update from ecogene.org as a multi-database
            collaboration
REFERENCE   16 (bases 1 to 840)
  AUTHORS   Rudd,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2008) Department of Biochemistry and Molecular
            Biology, University of Miami Miller School of Medicine, 118 Gautier
            Bldg., Miami, FL 33136, USA
  REMARK    Annotation update from ecogene.org as a multi-database
            collaboration
REFERENCE   17 (bases 1 to 840)
  AUTHORS   Rudd,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2008) Department of Biochemistry and Molecular
            Biology, University of Miami Miller School of Medicine, 118 Gautier
            Bldg., Miami, FL 33136, USA
  REMARK    Annotation update from ecogene.org as a multi-database
            collaboration
COMMENT     On or before Jun 21, 2004 this sequence version replaced
            gi:1786181, gi:1786192, gi:2367095, gi:1786217, gi:1786230,
            gi:1786240, gi:1786250, gi:1786262, gi:1786283, gi:1786298,
            gi:1786306, gi:1786315, gi:1786327, gi:1786339, gi:1786348,
            gi:1786358, gi:1786370, gi:1786383, gi:1786395, gi:1786402,
            gi:1786415, gi:2367098, gi:2367099, gi:1786454, gi:1786465,
            gi:2367103, gi:2367108, gi:1786501, gi:1786510, gi:1786520,
            gi:1786532, gi:1786542, gi:1786554, gi:1786568, gi:1786580,
            gi:1786596, gi:1786603, gi:1786614, gi:1786628, gi:1786639,
            gi:1786649, gi:1786660, gi:1786671, gi:1786683, gi:1786692,
            gi:1786705, gi:1786716, gi:1786728, gi:1786739, gi:1786751,
            gi:1786766, gi:1786782, gi:1786790, gi:1786800, gi:1786808,
            gi:1786819, gi:1786836, gi:1786849, gi:1786862, gi:1786875,
            gi:1786888, gi:1786896, gi:1786910, gi:1786920, gi:1786934,
            gi:1786947, gi:1786955, gi:1786967, gi:1786978, gi:1786988,
            gi:1786998, gi:1787015, gi:1787025, gi:1787036, gi:1787047,
            gi:1787058, gi:1787070, gi:1787084, gi:1787097, gi:1787106,
            gi:1787115, gi:1787125, gi:1787134, gi:1787148, gi:1787156,
            gi:1787169, gi:1787180, gi:1787189, gi:1787202, gi:2367111,
            gi:2367113, gi:1787233, gi:1787248, gi:1787256, gi:1787265,
            gi:1787282, gi:1787293, gi:1787308, gi:1787322, gi:1787332,
            gi:1787345, gi:1787358, gi:1787371, gi:1787382, gi:1787405,
            gi:1787417, gi:1787434, gi:1787444, gi:1787453, gi:1787467,
            gi:1787476, gi:1787486, gi:1787496, gi:1787509, gi:1787523,
            gi:2367115, gi:1787543, gi:2367117, gi:1787566, gi:1787578,
            gi:1787588, gi:1787600, gi:1787613, gi:1787633, gi:1787643,
            gi:1787652, gi:1787665, gi:1787673, gi:1787682, gi:1787695,
            gi:1787706, gi:1787720, gi:1787730, gi:1787742, gi:1787752,
            gi:1787764, gi:1787773, gi:1787783, gi:1787790, gi:1787801,
            gi:1787814, gi:2367119, gi:1787841, gi:1787862, gi:1787875,
            gi:1787888, gi:1787898, gi:2367121, gi:1787921, gi:1787935,
            gi:1787945, gi:1787955, gi:1787966, gi:1787978, gi:2367122,
            gi:1787997, gi:1788011, gi:1788022, gi:1788033, gi:1788045,
            gi:1788058, gi:1788067, gi:1788078, gi:1788089, gi:1788106,
            gi:1788117, gi:1788129, gi:1788139, gi:1788154, gi:1788163,
            gi:1788179, gi:1788189, gi:1788200, gi:1788214, gi:1788229,
            gi:1788241, gi:1788257, gi:2367124, gi:1788285, gi:2367125,
            gi:1788298, gi:1788310, gi:2367127, gi:1788338, gi:1788354,
            gi:1788373, gi:1788382, gi:1788395, gi:1788413, gi:1788425,
            gi:2367129, gi:1788447, gi:1788456, gi:1788470, gi:1788479,
            gi:1788489, gi:1788498, gi:1788508, gi:1788520, gi:2367131,
            gi:1788547, gi:1788555, gi:2367132, gi:1788570, gi:1788582,
            gi:1788594, gi:1788605, gi:1788623, gi:1788634, gi:1788647,
            gi:1788659, gi:1788672, gi:1788684, gi:1788694, gi:1788709,
            gi:1788718, gi:1788731, gi:2367135, gi:2367137, gi:1788763,
            gi:1788775, gi:1788789, gi:1788805, gi:1788813, gi:1788821,
            gi:1788839, gi:1788850, gi:1788862, gi:1788870, gi:1788883,
            gi:1788899, gi:1788907, gi:2367139, gi:1788927, gi:1788939,
            gi:2367141, gi:2367142, gi:1788975, gi:2367143, gi:2367147,
            gi:1789011, gi:1789024, gi:1789037, gi:2367149, gi:2367151,
            gi:2367153, gi:2367155, gi:2367156, gi:1789110, gi:2367157,
            gi:2367160, gi:1789143, gi:1789153, gi:2367162, gi:2367163,
            gi:1789185, gi:1789195, gi:2367165, gi:2367168, gi:2367170,
            gi:1789239, gi:2367171, gi:2367173, gi:1789270, gi:1789282,
            gi:2367176, gi:2367178, gi:1789319, gi:2367179, gi:1789344,
            gi:2367181, gi:2367182, gi:2367184, gi:2367186, gi:1789405,
            gi:2367187, gi:1789431, gi:1789441, gi:1789451, gi:2367189,
            gi:2367191, gi:2367194, gi:1789499, gi:2367197, gi:1789524,
            gi:1789536, gi:2367199, gi:1789562, gi:2367201, gi:2367203,
            gi:1789607, gi:1789619, gi:2367205, gi:2367207, gi:1789659,
            gi:2367209, gi:2367211, gi:1789694, gi:1789718, gi:1789734,
            gi:2367213, gi:1789758, gi:2367215, gi:1789783, gi:1789798,
            gi:2367219, gi:2367220, gi:2367222, gi:2367227, gi:1789840,
            gi:1789854, gi:1789868, gi:1789880, gi:2367230, gi:2367232,
            gi:1789910, gi:1789919, gi:1789931, gi:2367235, gi:2367238,
            gi:1789957, gi:2367241, gi:1789977, gi:1789989, gi:2367244,
            gi:2367246, gi:2367249, gi:2367251, gi:1790036, gi:2367252,
            gi:1790063, gi:2367253, gi:2367255, gi:2367258, gi:1790105,
            gi:2367261, gi:2367266, gi:1790142, gi:2367269, gi:1790166,
            gi:2367272, gi:1790188, gi:2367276, gi:2367278, gi:2367282,
            gi:2367291, gi:2367294, gi:2367299, gi:2367306, gi:2367315,
            gi:2367318, gi:1790295, gi:2367320, gi:2367324, gi:2367326,
            gi:2367328, gi:1790356, gi:1790374, gi:1790385, gi:2367332,
            gi:1790404, gi:2367333, gi:2367336, gi:1790440, gi:1790448,
            gi:1790456, gi:2367338, gi:2367339, gi:2367340, gi:2367344,
            gi:2367346, gi:2367349, gi:2367351, gi:2367352, gi:1790563,
            gi:1790574, gi:1790582, gi:2367354, gi:1790607, gi:2367356,
            gi:2367357, gi:1790649, gi:2367360, gi:1790670, gi:2367361,
            gi:2367366, gi:1790711, gi:2367368, gi:1790732, gi:2367369,
            gi:2367372, gi:2367374, gi:1790777, gi:1790789, gi:2367375,
            gi:2367377, gi:2367380, gi:2367382, gi:2367383, gi:1790858,
            gi:6626251.
            Current U00096 annotation updates are derived from EcoGene
            http://ecogene.org. Suggestions for updates can be sent to Dr.
            Kenneth Rudd (krudd@miami.edu). These updates are being generated
            from a collaboration that also includes ASAP/ERIC, the Coli Genetic
            Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Escherichia coli str. K-12 substr. MG1655"
                     /mol_type="genomic DNA"
                     /strain="K-12"
                     /sub_strain="MG1655"
                     /db_xref="taxon:511145"
     gene            190..255
                     /gene="thrL"
                     /locus_tag="b0001"
                     /gene_synonym="ECK0001"
                     /gene_synonym="JW4367"
                     /db_xref="EcoGene:EG11277"
     CDS             190..255
                     /gene="thrL"
                     /locus_tag="b0001"
                     /gene_synonym="ECK0001"
                     /gene_synonym="JW4367"
                     /function="leader; Amino acid biosynthesis: Threonine"
                     /function="1.5.1.8 metabolism; building block
                     biosynthesis; amino acids; threonine"
                     /note="GO_process: GO:0009088 - threonine biosynthetic
                     process"
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="AAC73112.1"
                     /db_xref="GI:1786182"
                     /db_xref="ASAP:ABE-0000006"
                     /db_xref="UniProtKB/Swiss-Prot:P0AD86"
                     /db_xref="EcoGene:EG11277"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            337..>840
                     /gene="thrA"
                     /locus_tag="b0002"
                     /gene_synonym="ECK0002"
                     /gene_synonym="Hs"
                     /gene_synonym="JW0001"
                     /gene_synonym="thrA1"
                     /gene_synonym="thrA2"
                     /gene_synonym="thrD"
                     /db_xref="EcoGene:EG10998"
     CDS             337..>840
                     /gene="thrA"
                     /locus_tag="b0002"
                     /gene_synonym="ECK0002"
                     /gene_synonym="Hs"
                     /gene_synonym="JW0001"
                     /gene_synonym="thrA1"
                     /gene_synonym="thrA2"
                     /gene_synonym="thrD"
                     /EC_number="2.7.2.4"
                     /EC_number="1.1.1.3"
                     /function="enzyme; Amino acid biosynthesis: Threonine"
                     /function="1.5.1.8 metabolism; building block
                     biosynthesis; amino acids; threonine"
                     /function="1.5.1.21 metabolism; building block
                     biosynthesis; amino acids; homoserine"
                     /function="7.1 location of gene products; cytoplasm"
                     /experiment="N-terminus verified by Edman degradation:
                     PMID 354697,4562989"
                     /note="bifunctional: aspartokinase I (N-terminal);
                     homoserine dehydrogenase I (C-terminal);
                     GO_component: GO:0005737 - cytoplasm;
                     GO_process: GO:0009088 - threonine biosynthetic process;
                     GO_process: GO:0009086 - methionine biosynthetic process;
                     GO_process: GO:0009090 - homoserine biosynthetic process"
                     /codon_start=1
                     /transl_table=11
                     /product="fused aspartokinase I and homoserine
                     dehydrogenase I"
                     /protein_id="AAC73113.1"
                     /db_xref="GI:1786183"
                     /db_xref="ASAP:ABE-0000008"
                     /db_xref="UniProtKB/Swiss-Prot:P00561"
                     /db_xref="EcoGene:EG10998"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
ORIGIN      
        1 agcttttcat tctgactgca acgggcaata tgtctctgtg tggattaaaa aaagagtgtc
       61 tgatagcagc ttctgaactg gttacctgcc gtgagtaaat taaaatttta ttgacttagg
      121 tcactaaata ctttaaccaa tataggcata gcgcacagac agataaaaat tacagagtac
      181 acaacatcca tgaaacgcat tagcaccacc attaccacca ccatcaccat taccacaggt
      241 aacggtgcgg gctgacgcgt acaggaaaca cagaaaaaag cccgcacctg acagtgcggg
      301 cttttttttt cgaccaaagg taacgaggta acaaccatgc gagtgttgaa gttcggcggt
      361 acatcagtgg caaatgcaga acgttttctg cgtgttgccg atattctgga aagcaatgcc
      421 aggcaggggc aggtggccac cgtcctctct gcccccgcca aaatcaccaa ccacctggtg
      481 gcgatgattg aaaaaaccat tagcggccag gatgctttac ccaatatcag cgatgccgaa
      541 cgtatttttg ccgaactttt gacgggactc gccgccgccc agccggggtt cccgctggcg
      601 caattgaaaa ctttcgtcga tcaggaattt gcccaaataa aacatgtcct gcatggcatt
      661 agtttgttgg ggcagtgccc ggatagcatc aacgctgcgc tgatttgccg tggcgagaaa
      721 atgtcgatcg ccattatggc cggcgtatta gaagcgcgcg gtcacaacgt tactgttatc
      781 gatccggtcg aaaaactgct ggcagtgggg cattacctcg aatctaccgt cgatattgct
//
LOCUS       U00096                   910 bp    DNA     linear   BCT 11-MAR-2009
DEFINITION  Escherichia coli str. K-12 substr. MG1655, complete genome.
ACCESSION   U00096 REGION: 1..910
VERSION     U00096.2  GI:48994873
PROJECT     GenomeProject:225
DBLINK      Project:225
KEYWORDS    .
SOURCE      Escherichia coli str. K-12 substr. MG1655
  ORGANISM  Escherichia coli str. K-12 substr. MG1655
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (sites)
  AUTHORS   Kang,W.K., Icho,T., Isono,S., Kitakawa,M. and Isono,K.
  TITLE     Characterization of the gene rimK responsible for the addition of
            glutamic acid residues to the C-terminus of ribosomal protein S6 in
            Escherichia coli K12
  JOURNAL   Mol. Gen. Genet. 217 (2-3), 281-288 (1989)
   PUBMED   2570347
REFERENCE   2  (bases 1 to 910)
  AUTHORS   Blattner,F.R., Plunkett,G., Bloch,C.A., Perna,N.T., Burland,V.,
            Riley,M., Collado-Vides,J., Glasner,J.D., Rode,C.K., Mayhew,G.F.,
            Gregor,J., Davis,N.W., Kirkpatrick,H.A., Goeden,M.A., Rose,D.J.,
            Mau,B. and Shao,Y.
  TITLE     The complete genome sequence of Escherichia coli K-12
  JOURNAL   Science 277 (5331), 1453-1474 (1997)
   PUBMED   9278503
REFERENCE   3  (bases 1 to 910)
  AUTHORS   Riley,M., Abe,T., Arnaud,M.B., Berlyn,M.K., Blattner,F.R.,
            Chaudhuri,R.R., Glasner,J.D., Horiuchi,T., Keseler,I.M., Kosuge,T.,
            Mori,H., Perna,N.T., Plunkett,G. III, Rudd,K.E., Serres,M.H.,
            Thomas,G.H., Thomson,N.R., Wishart,D. and Wanner,B.L.
  TITLE     Escherichia coli K-12: a cooperatively developed annotation
            snapshot--2005
  JOURNAL   Nucleic Acids Res. 34 (1), 1-9 (2006)
   PUBMED   16397293
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 910)
  AUTHORS   Arnaud,M., Berlyn,M.K.B., Blattner,F.R., Galperin,M.Y.,
            Glasner,J.D., Horiuchi,T., Kosuge,T., Mori,H., Perna,N.T.,
            Plunkett,G. III, Riley,M., Rudd,K.E., Serres,M.H., Thomas,G.H. and
            Wanner,B.L.
  TITLE     Workshop on Annotation of Escherichia coli K-12
  JOURNAL   Unpublished
  REMARK    Woods Hole, Mass., on 14-18 November 2003 (sequence corrections)
REFERENCE   5  (bases 1 to 910)
  AUTHORS   Glasner,J.D., Perna,N.T., Plunkett,G. III, Anderson,B.D.,
            Bockhorst,J., Hu,J.C., Riley,M., Rudd,K.E. and Serres,M.H.
  TITLE     ASAP: Escherichia coli K-12 strain MG1655 version m56
  JOURNAL   Unpublished
  REMARK    ASAP download 10 June 2004 (annotation updates)
REFERENCE   6  (bases 1 to 910)
  AUTHORS   Hayashi,K., Morooka,N., Mori,H. and Horiuchi,T.
  TITLE     A more accurate sequence comparison between genomes of Escherichia
            coli K12 W3110 and MG1655 strains
  JOURNAL   Unpublished
  REMARK    GenBank accessions AG613214 to AG613378 (sequence corrections)
REFERENCE   7  (bases 1 to 910)
  AUTHORS   Perna,N.T.
  TITLE     Escherichia coli K-12 MG1655 yqiK-rfaE intergenic region, genomic
            sequence correction
  JOURNAL   Unpublished
  REMARK    GenBank accession AY605712 (sequence corrections)
REFERENCE   8  (bases 1 to 910)
  AUTHORS   Rudd,K.E.
  TITLE     A manual approach to accurate translation start site annotation: an
            E. coli K-12 case study
  JOURNAL   Unpublished
REFERENCE   9  (bases 1 to 910)
  AUTHORS   Blattner,F.R. and Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-1997) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
REFERENCE   10 (bases 1 to 910)
  AUTHORS   Blattner,F.R. and Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-1997) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
REFERENCE   11 (bases 1 to 910)
  AUTHORS   Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-1998) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
REFERENCE   12 (bases 1 to 910)
  AUTHORS   Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2004) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
  REMARK    Sequence update by submitter
REFERENCE   13 (bases 1 to 910)
  AUTHORS   Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2006) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
  REMARK    Protein updates by submitter
REFERENCE   14 (bases 1 to 910)
  AUTHORS   Rudd,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2007) Department of Biochemistry and Molecular
            Biology, University of Miami Miller School of Medicine, 118 Gautier
            Bldg., Miami, FL 33136, USA
  REMARK    Annotation update from ecogene.org as a multi-database
            collaboration
REFERENCE   15 (bases 1 to 910)
  AUTHORS   Rudd,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2007) Department of Biochemistry and Molecular
            Biology, University of Miami Miller School of Medicine, 118 Gautier
            Bldg., Miami, FL 33136, USA
  REMARK    Annotation update from ecogene.org as a multi-database
            collaboration
REFERENCE   16 (bases 1 to 910)
  AUTHORS   Rudd,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2008) Department of Biochemistry and Molecular
            Biology, University of Miami Miller School of Medicine, 118 Gautier
            Bldg., Miami, FL 33136, USA
  REMARK    Annotation update from ecogene.org as a multi-database
            collaboration
REFERENCE   17 (bases 1 to 910)
  AUTHORS   Rudd,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2008) Department of Biochemistry and Molecular
            Biology, University of Miami Miller School of Medicine, 118 Gautier
            Bldg., Miami, FL 33136, USA
  REMARK    Annotation update from ecogene.org as a multi-database
            collaboration
COMMENT     On or before Jun 21, 2004 this sequence version replaced
            gi:1786181, gi:1786192, gi:2367095, gi:1786217, gi:1786230,
            gi:1786240, gi:1786250, gi:1786262, gi:1786283, gi:1786298,
            gi:1786306, gi:1786315, gi:1786327, gi:1786339, gi:1786348,
            gi:1786358, gi:1786370, gi:1786383, gi:1786395, gi:1786402,
            gi:1786415, gi:2367098, gi:2367099, gi:1786454, gi:1786465,
            gi:2367103, gi:2367108, gi:1786501, gi:1786510, gi:1786520,
            gi:1786532, gi:1786542, gi:1786554, gi:1786568, gi:1786580,
            gi:1786596, gi:1786603, gi:1786614, gi:1786628, gi:1786639,
            gi:1786649, gi:1786660, gi:1786671, gi:1786683, gi:1786692,
            gi:1786705, gi:1786716, gi:1786728, gi:1786739, gi:1786751,
            gi:1786766, gi:1786782, gi:1786790, gi:1786800, gi:1786808,
            gi:1786819, gi:1786836, gi:1786849, gi:1786862, gi:1786875,
            gi:1786888, gi:1786896, gi:1786910, gi:1786920, gi:1786934,
            gi:1786947, gi:1786955, gi:1786967, gi:1786978, gi:1786988,
            gi:1786998, gi:1787015, gi:1787025, gi:1787036, gi:1787047,
            gi:1787058, gi:1787070, gi:1787084, gi:1787097, gi:1787106,
            gi:1787115, gi:1787125, gi:1787134, gi:1787148, gi:1787156,
            gi:1787169, gi:1787180, gi:1787189, gi:1787202, gi:2367111,
            gi:2367113, gi:1787233, gi:1787248, gi:1787256, gi:1787265,
            gi:1787282, gi:1787293, gi:1787308, gi:1787322, gi:1787332,
            gi:1787345, gi:1787358, gi:1787371, gi:1787382, gi:1787405,
            gi:1787417, gi:1787434, gi:1787444, gi:1787453, gi:1787467,
            gi:1787476, gi:1787486, gi:1787496, gi:1787509, gi:1787523,
            gi:2367115, gi:1787543, gi:2367117, gi:1787566, gi:1787578,
            gi:1787588, gi:1787600, gi:1787613, gi:1787633, gi:1787643,
            gi:1787652, gi:1787665, gi:1787673, gi:1787682, gi:1787695,
            gi:1787706, gi:1787720, gi:1787730, gi:1787742, gi:1787752,
            gi:1787764, gi:1787773, gi:1787783, gi:1787790, gi:1787801,
            gi:1787814, gi:2367119, gi:1787841, gi:1787862, gi:1787875,
            gi:1787888, gi:1787898, gi:2367121, gi:1787921, gi:1787935,
            gi:1787945, gi:1787955, gi:1787966, gi:1787978, gi:2367122,
            gi:1787997, gi:1788011, gi:1788022, gi:1788033, gi:1788045,
            gi:1788058, gi:1788067, gi:1788078, gi:1788089, gi:1788106,
            gi:1788117, gi:1788129, gi:1788139, gi:1788154, gi:1788163,
            gi:1788179, gi:1788189, gi:1788200, gi:1788214, gi:1788229,
            gi:1788241, gi:1788257, gi:2367124, gi:1788285, gi:2367125,
            gi:1788298, gi:1788310, gi:2367127, gi:1788338, gi:1788354,
            gi:1788373, gi:1788382, gi:1788395, gi:1788413, gi:1788425,
            gi:2367129, gi:1788447, gi:1788456, gi:1788470, gi:1788479,
            gi:1788489, gi:1788498, gi:1788508, gi:1788520, gi:2367131,
            gi:1788547, gi:1788555, gi:2367132, gi:1788570, gi:1788582,
            gi:1788594, gi:1788605, gi:1788623, gi:1788634, gi:1788647,
            gi:1788659, gi:1788672, gi:1788684, gi:1788694, gi:1788709,
            gi:1788718, gi:1788731, gi:2367135, gi:2367137, gi:1788763,
            gi:1788775, gi:1788789, gi:1788805, gi:1788813, gi:1788821,
            gi:1788839, gi:1788850, gi:1788862, gi:1788870, gi:1788883,
            gi:1788899, gi:1788907, gi:2367139, gi:1788927, gi:1788939,
            gi:2367141, gi:2367142, gi:1788975, gi:2367143, gi:2367147,
            gi:1789011, gi:1789024, gi:1789037, gi:2367149, gi:2367151,
            gi:2367153, gi:2367155, gi:2367156, gi:1789110, gi:2367157,
            gi:2367160, gi:1789143, gi:1789153, gi:2367162, gi:2367163,
            gi:1789185, gi:1789195, gi:2367165, gi:2367168, gi:2367170,
            gi:1789239, gi:2367171, gi:2367173, gi:1789270, gi:1789282,
            gi:2367176, gi:2367178, gi:1789319, gi:2367179, gi:1789344,
            gi:2367181, gi:2367182, gi:2367184, gi:2367186, gi:1789405,
            gi:2367187, gi:1789431, gi:1789441, gi:1789451, gi:2367189,
            gi:2367191, gi:2367194, gi:1789499, gi:2367197, gi:1789524,
            gi:1789536, gi:2367199, gi:1789562, gi:2367201, gi:2367203,
            gi:1789607, gi:1789619, gi:2367205, gi:2367207, gi:1789659,
            gi:2367209, gi:2367211, gi:1789694, gi:1789718, gi:1789734,
            gi:2367213, gi:1789758, gi:2367215, gi:1789783, gi:1789798,
            gi:2367219, gi:2367220, gi:2367222, gi:2367227, gi:1789840,
            gi:1789854, gi:1789868, gi:1789880, gi:2367230, gi:2367232,
            gi:1789910, gi:1789919, gi:1789931, gi:2367235, gi:2367238,
            gi:1789957, gi:2367241, gi:1789977, gi:1789989, gi:2367244,
            gi:2367246, gi:2367249, gi:2367251, gi:1790036, gi:2367252,
            gi:1790063, gi:2367253, gi:2367255, gi:2367258, gi:1790105,
            gi:2367261, gi:2367266, gi:1790142, gi:2367269, gi:1790166,
            gi:2367272, gi:1790188, gi:2367276, gi:2367278, gi:2367282,
            gi:2367291, gi:2367294, gi:2367299, gi:2367306, gi:2367315,
            gi:2367318, gi:1790295, gi:2367320, gi:2367324, gi:2367326,
            gi:2367328, gi:1790356, gi:1790374, gi:1790385, gi:2367332,
            gi:1790404, gi:2367333, gi:2367336, gi:1790440, gi:1790448,
            gi:1790456, gi:2367338, gi:2367339, gi:2367340, gi:2367344,
            gi:2367346, gi:2367349, gi:2367351, gi:2367352, gi:1790563,
            gi:1790574, gi:1790582, gi:2367354, gi:1790607, gi:2367356,
            gi:2367357, gi:1790649, gi:2367360, gi:1790670, gi:2367361,
            gi:2367366, gi:1790711, gi:2367368, gi:1790732, gi:2367369,
            gi:2367372, gi:2367374, gi:1790777, gi:1790789, gi:2367375,
            gi:2367377, gi:2367380, gi:2367382, gi:2367383, gi:1790858,
            gi:6626251.
            Current U00096 annotation updates are derived from EcoGene
            http://ecogene.org. Suggestions for updates can be sent to Dr.
            Kenneth Rudd (krudd@miami.edu). These updates are being generated
            from a collaboration that also includes ASAP/ERIC, the Coli Genetic
            Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Escherichia coli str. K-12 substr. MG1655"
                     /mol_type="genomic DNA"
                     /strain="K-12"
                     /sub_strain="MG1655"
                     /db_xref="taxon:511145"
     gene            190..255
                     /gene="thrL"
                     /locus_tag="b0001"
                     /gene_synonym="ECK0001"
                     /gene_synonym="JW4367"
                     /db_xref="EcoGene:EG11277"
     CDS             190..255
                     /gene="thrL"
                     /locus_tag="b0001"
                     /gene_synonym="ECK0001"
                     /gene_synonym="JW4367"
                     /function="leader; Amino acid biosynthesis: Threonine"
                     /function="1.5.1.8 metabolism; building block
                     biosynthesis; amino acids; threonine"
                     /note="GO_process: GO:0009088 - threonine biosynthetic
                     process"
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="AAC73112.1"
                     /db_xref="GI:1786182"
                     /db_xref="ASAP:ABE-0000006"
                     /db_xref="UniProtKB/Swiss-Prot:P0AD86"
                     /db_xref="EcoGene:EG11277"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            337..>910
                     /gene="thrA"
                     /locus_tag="b0002"
                     /gene_synonym="ECK0002"
                     /gene_synonym="Hs"
                     /gene_synonym="JW0001"
                     /gene_synonym="thrA1"
                     /gene_synonym="thrA2"
                     /gene_synonym="thrD"
                     /db_xref="EcoGene:EG10998"
     CDS             337..>910
                     /gene="thrA"
                     /locus_tag="b0002"
                     /gene_synonym="ECK0002"
                     /gene_synonym="Hs"
                     /gene_synonym="JW0001"
                     /gene_synonym="thrA1"
                     /gene_synonym="thrA2"
                     /gene_synonym="thrD"
                     /EC_number="2.7.2.4"
                     /EC_number="1.1.1.3"
                     /function="enzyme; Amino acid biosynthesis: Threonine"
                     /function="1.5.1.8 metabolism; building block
                     biosynthesis; amino acids; threonine"
                     /function="1.5.1.21 metabolism; building block
                     biosynthesis; amino acids; homoserine"
                     /function="7.1 location of gene products; cytoplasm"
                     /experiment="N-terminus verified by Edman degradation:
                     PMID 354697,4562989"
                     /note="bifunctional: aspartokinase I (N-terminal);
                     homoserine dehydrogenase I (C-terminal);
                     GO_component: GO:0005737 - cytoplasm;
                     GO_process: GO:0009088 - threonine biosynthetic process;
                     GO_process: GO:0009086 - methionine biosynthetic process;
                     GO_process: GO:0009090 - homoserine biosynthetic process"
                     /codon_start=1
                     /transl_table=11
                     /product="fused aspartokinase I and homoserine
                     dehydrogenase I"
                     /protein_id="AAC73113.1"
                     /db_xref="GI:1786183"
                     /db_xref="ASAP:ABE-0000008"
                     /db_xref="UniProtKB/Swiss-Prot:P00561"
                     /db_xref="EcoGene:EG10998"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
ORIGIN      
        1 agcttttcat tctgactgca acgggcaata tgtctctgtg tggattaaaa aaagagtgtc
       61 tgatagcagc ttctgaactg gttacctgcc gtgagtaaat taaaatttta ttgacttagg
      121 tcactaaata ctttaaccaa tataggcata gcgcacagac agataaaaat tacagagtac
      181 acaacatcca tgaaacgcat tagcaccacc attaccacca ccatcaccat taccacaggt
      241 aacggtgcgg gctgacgcgt acaggaaaca cagaaaaaag cccgcacctg acagtgcggg
      301 cttttttttt cgaccaaagg taacgaggta acaaccatgc gagtgttgaa gttcggcggt
      361 acatcagtgg caaatgcaga acgttttctg cgtgttgccg atattctgga aagcaatgcc
      421 aggcaggggc aggtggccac cgtcctctct gcccccgcca aaatcaccaa ccacctggtg
      481 gcgatgattg aaaaaaccat tagcggccag gatgctttac ccaatatcag cgatgccgaa
      541 cgtatttttg ccgaactttt gacgggactc gccgccgccc agccggggtt cccgctggcg
      601 caattgaaaa ctttcgtcga tcaggaattt gcccaaataa aacatgtcct gcatggcatt
      661 agtttgttgg ggcagtgccc ggatagcatc aacgctgcgc tgatttgccg tggcgagaaa
      721 atgtcgatcg ccattatggc cggcgtatta gaagcgcgcg gtcacaacgt tactgttatc
      781 gatccggtcg aaaaactgct ggcagtgggg cattacctcg aatctaccgt cgatattgct
      841 gagtccaccc gccgtattgc ggcaagccgc attccggctg atcacatggt gctgatggca
      901 ggtttcaccg
//
